Regulatory

Part:BBa_K4307000:Design

Designed by: Chi Zhang   Group: iGEM22_Tsinghua   (2022-09-27)


OR1-OR2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Comparing the fluorescence data, it can be found that when we test OR1-OR2 as a promoter alone, a significant fluorescence increment can be observed at 16℃ and 37℃ compared with negative Control. Further comparison of the fluorescence intensity before and after the introduction of cI protein indicates that OR1-OR2 can be significantly inhibited by cI protein.


Source

Lambda bacterial phage

References